Authors:Christopher Philip Mauer (cpmauer at northwestern.edu)

Vytas Bindokas (vytas at drugs.bsd.uchicago.edu)History:2004/07/15: First version

2007/10/26: Fixed batch mode NullPointerException bug

Limitations:Only works with 8-bit and 16-bit images Source:Hybrid_3D_Median_Filter.java Installation:Download Hybrid_3D_Median_Filter.class to the plugins folder, restart ImageJ, and there will be a new "Hybrid Median Filter" command in the Plugins menu. Description:This plugin consists of a 3D adaptation of the Hybrid Median Filter intended for XYZ or XYT data, which we are calling a 3d Hybrid Median Filter. In this implementation, the median is calculated from the medians of 1) the 3x3 2d PLUS kernel, 2) the 3x3 2d X kernel, 3) the 3x3 3d PLUS kernel, and four 3x3 2d X kernels through the center pixel along Z-axis: 4) the vertical X, 5) the horizontal X, 6) the X from the upper right to the lower left, and 7) the X from the upper left to the lower right, and optionally 8) the pixel in question. The 3D median model is experimental, intended for series (time or finely-spaced z slices) where high noise places little confidence on individual pixel values per image. The pixel in question receives less weighing in medians (included in 7 medians calculations) versus checked (7 median ranks plus median of the 7 AND the pixel). Checking the box retains single pixel data more (see frame numbers or 1-pixel scratches), and performs less data "restoration". Since the filter samples local 3x3x3 volumes, data stacks should be oversampled. The top and bottom edge pixels are reflected outward, and the side edge pixels are wrapped arpund to complete the edge bound kernels. The pixels of the first and last slice are reflected outward to simulate data for the N-1 and N+1 slices (where N is the number of images in the stack) in order to return an output stack which is the same size as the input stack. The filter may be aborted by hitting the escape key.

See 2d Hybrid Median Filter for a version which works on single images and slices of a stack independent of one another.

Copyright (C) 2004 CHRISTOPHER PHILIP MAUER and VYTAS BINDOKAS.

Permission to use, copy, modify, and distribute this software for any purpose without fee is hereby granted, provided that this entire notice is included in all copies of any software which is or includes a copy or modification of this software and in all copies of the supporting documentation for such software. Any for profit use of this software is expressly forbidden without first obtaining the explicit consent of the author.

THIS SOFTWARE IS BEING PROVIDED "AS IS", WITHOUT ANY EXPRESS OR IMPLIED WARRANTY. IN PARTICULAR, THE AUTHOR DOES NOT MAKE ANY REPRESENTATION OR WARRANTY OF ANY KIND CONCERNING THE MERCHANTABILITY OF THIS SOFTWARE OR ITS FITNESS FOR ANY PARTICULAR PURPOSE

The plugin operates on a previously opened stack of images, and produces a new stack of filtered images. The output stack is generated upon completion of processing for all of the input data. Be sure to save the output if desired.

Figure 1) 3d Hybrid Median Filter Demo

The lefthand frame represents the original data, the middle is filtered data excluding the pixel in question in the final median (unchecked option), and the righthand side is the filtered data including the pixel in question (checked option). Playback on key frames is slowed to allow appreciation of intended effects and event description. This aniGif is a high speed, low light stream of Fluo-4 in chick cardiomyocytes using a CARV spinning disk confocal and an EMCDD camera. Credits MW Roe and P Schumacker UofC.