Command Shortcut Description New N Creates a new image or stack Open O Opens a TIFF, GIF, JPEG, BMP, DICOM or FITS Open Samples Shift-B Opens "Blobs" example image Close W Closes the active window Save S Saves active image in Tiff format Revert R Revert to saved version of image P Print active image Undo Z Reverses the effect of the last operation Cut X Copy image to clipboard and clear selection Copy C Copy image to clipboard Paste V Paste clipboard selection into active image Clear backspace Erases selection to background color Select All A Select entire image Select None Shift-A Delete selection Restore Selection Shift-E Restores ROI Fill F Fills selection in foreground color Draw D Draw selection Invert Shift-I Invert image or selection Adjust Contrast Shift-C Adjust brightness and contrast Adjust Threshold Shift-T Adjust threshold levels Show Info I Displays information about active image Next Slice > Advance to next stack slice Previous Slice < Backup up to previous stack slice Start Animation = Starts/stops stack animation Duplicate Shift-D Duplicates active image or selection Scale E Scale image or selection Smooth Shift-S 3x3 unweighted smoothing Find Edges Shift-F Performs Sobel edge detection Repeat Command Shift-R Repeats previous command Measure M Displays statistics about active image or selection Histogram H Displays a histogram of the active window or selection Plot Profile K Displays density profile plot of current selection ImageJ enter Brings ImageJ window to front Put Behind tab Switches to next image window
Alt Key Modifications
Image/Adjust/Threshold: Adjusting Min also adjusts Max
Image/Stacks/Add Slice: Insert before current slice
Image/Stacks/Next Slice: Skip nine slices
Image/Stacks/Previous Slice: Skip nine slices
Image/Duplicate: Don't show dialog
Image/Colors: Alt-click to "pick up" background color
Process/Equalize: Do classic histogram equalization
Process/Subtract Background: Show background image
Analyze/Plot Profile: For rectangular selections, generate row average plots
Analyze/Tools/Analyze Line Graph: Show intermediate image
Plugins/Utilities/ImageJ Properties: List all Java properties
Plugins/Utilities/Monitor Memory: Simulate 640x480 frame grabber
Any User Plugin: Load using new class loader
Space Bar Modifications
Any Tool: Temporarily switch to the "hand" (scrolling) tool
Moving through a stack: Automatically adjust min/max display values
Arrow Keys
Use the arrow keys to move selection outlines one pixel at a time. Resize rectangular and oval selections by holding down the the alt (option) and while using the arrow keys.