This is a 16-bits/channel composite color image of HeLa cells with red lysosomes, green mitochondria and blue nucleus. Image courtesy of Tony Collins, creator of the ImageJ for Microscopy collection of plugins at . The scale of 19.42 pixels per micrometer is not accurate. Use the slider under the image to select the channel to be processed or analyzed. Note how the color of the subtitle changes when you switch to a different channel. Open the "Channels" tool by pressing shift-z (Image>Color>Channels Tool) to enable and disable channels. Use the B&C tool (press shift-c) to adjust the brightness and contrast of the current channel. Change the channel color by selecting a LUT from the Image>Lookup Tables menu. Press shift-f (Image>Overlay>Flatten) to convert to 24-bit RGB. Use File>Save As>Tiff to save as a 48-bit TIFF. The text in the lower right corner of the image is a non-destructive overlay that can be removed by using the Image>Overlay>Remove Overlay command. This text was copied to the image using the Edit>Copy to Image Info command in the ImageJ text editor.