// This macro creates a composite selection from a binary mask or // thresholded image. It runs the particle analyzer to find objects // in the mask, inverts the image so holes become objects, runs the // particle analyzer a second time, then creates a composite ROI // by adding the object outlines and subtracting the hole outlines. // In ImageJ 1.37 and later, create composite selections using // the Edit>Selection>Create Selection command, which is // much faster than this macro. setBatchMode(true); run("Set Measurements...", "area perimeter circularity decimal=3"); run("Analyze Particles...", "size=1 circularity=0 show=Nothing clear record"); count1 = nResults; run("Invert"); run("Analyze Particles...", "size=1 circularity=0 show=Nothing record exclude"); run("Invert"); for (i=0; i