Calculate Objective Parameters

Author: Sebastian Rhode (Sebastian.Rhode at gmx.de)

History: 2008/09/10: First version
2008/10/02: Version 1.1
2010/06/22: Version 1.3

Source: Calc_Objective.java

Installation: Download Calc_Objective.class to the plugins folder and restart ImageJ.

Description: This plugin calculates parameters of interest for an objective lens. Required Input Parameters are:

Objective NA
Magnification
Focal length of Tube Lens [mm]
Wavelength [nm]
Refractive index n1 (immersion medium)
Refractive Index n2 (medium; 1.33 (water) or 1.36 (cytosol))
Desired Fill factor of back Aperture (Beam Diameter = 1/e2) [mm]
CCD Pixel Size [microns]
Output parameters are:

Focal length of objective [mm]
Angle for Total Internal reflection [degrees]
Maximum working Angle of objective [degrees]
Rayleigh criterion [nm]
Spot diameter (1/e) [nm] ~ 0.78 * lambda / NA
Spot diameter (1/e) [pixel]
Effective Pupil diameter of objective lens [mm]
Required beam diameter (1/e2) for Fill Factor = 1 [mm]
Required beam diameter (1/e2) for Fill Factor = Desired Fill factor [mm]
Pixel Size in Object Plane [nm]

Author:	Sebastian Rhode (Sebastian.Rhode at gmx.de)
History:	2008/09/10: First version 2008/10/02: Version 1.1 2010/06/22: Version 1.3
Source:	Calc_Objective.java
Installation:	Download Calc_Objective.class to the plugins folder and restart ImageJ.
Description:	This plugin calculates parameters of interest for an objective lens. Required Input Parameters are: Objective NA Magnification Focal length of Tube Lens [mm] Wavelength [nm] Refractive index n1 (immersion medium) Refractive Index n2 (medium; 1.33 (water) or 1.36 (cytosol)) Desired Fill factor of back Aperture (Beam Diameter = 1/e2) [mm] CCD Pixel Size [microns] Output parameters are: Focal length of objective [mm] Angle for Total Internal reflection [degrees] Maximum working Angle of objective [degrees] Rayleigh criterion [nm] Spot diameter (1/e) [nm] ~ 0.78 * lambda / NA Spot diameter (1/e) [pixel] Effective Pupil diameter of objective lens [mm] Required beam diameter (1/e2) for Fill Factor = 1 [mm] Required beam diameter (1/e2) for Fill Factor = Desired Fill factor [mm] Pixel Size in Object Plane [nm]