Background Subtraction and Image Normalization

Background Subtraction and Image Normalization

Author: Jeffrey Kuhn (jkuhn at ccwf.cc.utexas.edu)
The University of Texas at Austin
Ben Dubin-Thaler (bjd14 at columbia.edu)
Columbia University

History: 2000/08/22: First version
2006/02/16: Works with stacks

Limitations: Currently limited to 16-bit images

Source: Normalize_.java

API: Normalize_.html

Installation: Download Normalize_.class to the plugins folder and restart ImageJ.

Description: This plugin simplifies the task of background subtraction and image normalization given a brightfield image and/or a background image. This plugin is currently limited to 16-bit images, but could easily be extended to 8-bit, 32-bit, or even color images.
Possible normalization methods:
If both BRIGHT and DARK images are specified
IMAGE = scale * (IMAGE - DARK) / (BRIGHT - DARK)
If only BRIGHT image is specified
IMAGE = scale * IMAGE / BRIGHT
If only DARK image is specified

IMAGE = IMAGE - DARK

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Author:	Jeffrey Kuhn (jkuhn at ccwf.cc.utexas.edu) The University of Texas at Austin Ben Dubin-Thaler (bjd14 at columbia.edu) Columbia University
History:	2000/08/22: First version 2006/02/16: Works with stacks
Limitations:	Currently limited to 16-bit images
Source:	Normalize_.java
API:	Normalize_.html
Installation:	Download Normalize_.class to the plugins folder and restart ImageJ.
Description:	This plugin simplifies the task of background subtraction and image normalization given a brightfield image and/or a background image. This plugin is currently limited to 16-bit images, but could easily be extended to 8-bit, 32-bit, or even color images. Possible normalization methods: If both BRIGHT and DARK images are specified IMAGE = scale * (IMAGE - DARK) / (BRIGHT - DARK) If only BRIGHT image is specified IMAGE = scale * IMAGE / BRIGHT If only DARK image is specified IMAGE = IMAGE - DARK