// This macro demonstrates now to run the particle analyzer
// (with overlay) on separate images of a stack.

  run("T1 Head (2.4M, 16-bits)");
  setThreshold(150, 65535);
  setSlice(60);
  run("Analyze Particles...", "size=400 show=Overlay slice"); 
  setThreshold(250, 65535);
  setSlice(62); 
  run("Analyze Particles...", "size=400 show=Overlay slice");
  resetThreshold;
  Roi.remove;
  Overlay.setStrokeColor("red");
  Overlay.setStrokeWidth(1);
  Overlay.setLabelFontSize(12, "bold scale back");
  Overlay.setLabelColor("cyan");
  run("In [+]"); run("In [+]");