run("Make Composite");
Stack.setDisplayMode("grayscale");
run("Set Measurements...", "area mean integrated display redirect=None decimal=3");
run("Clear Results");
setSlice(1);
count();
setSlice(2);
count();

function count() {
   run("Subtract Background...", "rolling=10 slice");
   setAutoThreshold("MaxEntropy dark");
   run("Analyze Particles...", "size=0-° circularity=0.00-1.00 show=Nothing display exclude slice");
}